Part:BBa_I723132:Design Deleted

XylR coding region

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 643
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 466
  Illegal PstI site found at 643
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 86
  Illegal BglII site found at 1548
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 643
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 643
  Illegal NgoMIV site found at 252
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 739
  

Design Notes

Site directed mutagenesis required to remove PstI site for full use of suffix and prefix when assembling this part next to others. this Part does not exist on its own, but as a composite with its native promoter Pr

Source

Cloned from the TOL plasmid from Pseudomonas putida MT2.

References

Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.